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rmil-22  (R&D Systems)


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    Structured Review

    R&D Systems rmil-22
    ( A ) Representative Western blot of SI organoids treated with or without MP (10 μM) for 24 hours, followed by treatment with <t>rmIL-22</t> (5 ng/mL) for 2 hours. ( B – D ) Representative images and frequency and size of SI organoids cultured with or without MP and rmIL-22 (0.5 ng/mL) for 5 days ( n = 3–4 wells per group). Scale bars: 200 μm. ( E and F ) Representative images and size of human SI organoids cultured with or without MP (10 μM) and rhIL-22 (10 ng/mL) for 6 days ( n = 6 wells per group). Scale bars: 1,000 μm. ( G and H ) qPCR to determine Cdkn1a and Ccna2 expression in organoids cultured with or without MP (10 μM) and rmIL-22 (1 ng/mL) for 3 days ( n = 3 wells per group). ( I and J ) B6 mice treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day). Representative images and ileal crypt frequency and height on day 7 ( n = 8–12 independent sections per group). Scale bars: 50 μm. ( K – M ) WT B6 mice were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting 72 hours after TBI. Representative images, ileal crypt frequency and height, and Ki67 + cell frequency ( n = 8–23 independent sections per group) on day 7. Scale bars: 50 μm. ( N – Q ) B6-into-BALB/c transplantation of BM with or without T cells. Recipients were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting on day 7 after BMT. Ileal crypt frequency and height and Ki67 + and Olfm4 + cell frequency ( n = 14–31 independent sections per group) on day 14 after BMT. Scale bars: 50 μm. Data are representative of at least 2 independent experiments or were combined from 2 experiments ( K – Q ). * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed t test or 1-way ANOVA.
    Rmil 22, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rmil-22/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    rmil-22 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Corticosteroids impair epithelial regeneration in immune-mediated intestinal damage"

    Article Title: Corticosteroids impair epithelial regeneration in immune-mediated intestinal damage

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI155880

    ( A ) Representative Western blot of SI organoids treated with or without MP (10 μM) for 24 hours, followed by treatment with rmIL-22 (5 ng/mL) for 2 hours. ( B – D ) Representative images and frequency and size of SI organoids cultured with or without MP and rmIL-22 (0.5 ng/mL) for 5 days ( n = 3–4 wells per group). Scale bars: 200 μm. ( E and F ) Representative images and size of human SI organoids cultured with or without MP (10 μM) and rhIL-22 (10 ng/mL) for 6 days ( n = 6 wells per group). Scale bars: 1,000 μm. ( G and H ) qPCR to determine Cdkn1a and Ccna2 expression in organoids cultured with or without MP (10 μM) and rmIL-22 (1 ng/mL) for 3 days ( n = 3 wells per group). ( I and J ) B6 mice treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day). Representative images and ileal crypt frequency and height on day 7 ( n = 8–12 independent sections per group). Scale bars: 50 μm. ( K – M ) WT B6 mice were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting 72 hours after TBI. Representative images, ileal crypt frequency and height, and Ki67 + cell frequency ( n = 8–23 independent sections per group) on day 7. Scale bars: 50 μm. ( N – Q ) B6-into-BALB/c transplantation of BM with or without T cells. Recipients were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting on day 7 after BMT. Ileal crypt frequency and height and Ki67 + and Olfm4 + cell frequency ( n = 14–31 independent sections per group) on day 14 after BMT. Scale bars: 50 μm. Data are representative of at least 2 independent experiments or were combined from 2 experiments ( K – Q ). * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed t test or 1-way ANOVA.
    Figure Legend Snippet: ( A ) Representative Western blot of SI organoids treated with or without MP (10 μM) for 24 hours, followed by treatment with rmIL-22 (5 ng/mL) for 2 hours. ( B – D ) Representative images and frequency and size of SI organoids cultured with or without MP and rmIL-22 (0.5 ng/mL) for 5 days ( n = 3–4 wells per group). Scale bars: 200 μm. ( E and F ) Representative images and size of human SI organoids cultured with or without MP (10 μM) and rhIL-22 (10 ng/mL) for 6 days ( n = 6 wells per group). Scale bars: 1,000 μm. ( G and H ) qPCR to determine Cdkn1a and Ccna2 expression in organoids cultured with or without MP (10 μM) and rmIL-22 (1 ng/mL) for 3 days ( n = 3 wells per group). ( I and J ) B6 mice treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day). Representative images and ileal crypt frequency and height on day 7 ( n = 8–12 independent sections per group). Scale bars: 50 μm. ( K – M ) WT B6 mice were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting 72 hours after TBI. Representative images, ileal crypt frequency and height, and Ki67 + cell frequency ( n = 8–23 independent sections per group) on day 7. Scale bars: 50 μm. ( N – Q ) B6-into-BALB/c transplantation of BM with or without T cells. Recipients were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting on day 7 after BMT. Ileal crypt frequency and height and Ki67 + and Olfm4 + cell frequency ( n = 14–31 independent sections per group) on day 14 after BMT. Scale bars: 50 μm. Data are representative of at least 2 independent experiments or were combined from 2 experiments ( K – Q ). * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed t test or 1-way ANOVA.

    Techniques Used: Western Blot, Cell Culture, Expressing, Transplantation Assay



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    ( A ) Representative Western blot of SI organoids treated with or without MP (10 μM) for 24 hours, followed by treatment with <t>rmIL-22</t> (5 ng/mL) for 2 hours. ( B – D ) Representative images and frequency and size of SI organoids cultured with or without MP and rmIL-22 (0.5 ng/mL) for 5 days ( n = 3–4 wells per group). Scale bars: 200 μm. ( E and F ) Representative images and size of human SI organoids cultured with or without MP (10 μM) and rhIL-22 (10 ng/mL) for 6 days ( n = 6 wells per group). Scale bars: 1,000 μm. ( G and H ) qPCR to determine Cdkn1a and Ccna2 expression in organoids cultured with or without MP (10 μM) and rmIL-22 (1 ng/mL) for 3 days ( n = 3 wells per group). ( I and J ) B6 mice treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day). Representative images and ileal crypt frequency and height on day 7 ( n = 8–12 independent sections per group). Scale bars: 50 μm. ( K – M ) WT B6 mice were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting 72 hours after TBI. Representative images, ileal crypt frequency and height, and Ki67 + cell frequency ( n = 8–23 independent sections per group) on day 7. Scale bars: 50 μm. ( N – Q ) B6-into-BALB/c transplantation of BM with or without T cells. Recipients were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting on day 7 after BMT. Ileal crypt frequency and height and Ki67 + and Olfm4 + cell frequency ( n = 14–31 independent sections per group) on day 14 after BMT. Scale bars: 50 μm. Data are representative of at least 2 independent experiments or were combined from 2 experiments ( K – Q ). * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed t test or 1-way ANOVA.
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    ( A ) Representative Western blot of SI organoids treated with or without MP (10 μM) for 24 hours, followed by treatment with <t>rmIL-22</t> (5 ng/mL) for 2 hours. ( B – D ) Representative images and frequency and size of SI organoids cultured with or without MP and rmIL-22 (0.5 ng/mL) for 5 days ( n = 3–4 wells per group). Scale bars: 200 μm. ( E and F ) Representative images and size of human SI organoids cultured with or without MP (10 μM) and rhIL-22 (10 ng/mL) for 6 days ( n = 6 wells per group). Scale bars: 1,000 μm. ( G and H ) qPCR to determine Cdkn1a and Ccna2 expression in organoids cultured with or without MP (10 μM) and rmIL-22 (1 ng/mL) for 3 days ( n = 3 wells per group). ( I and J ) B6 mice treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day). Representative images and ileal crypt frequency and height on day 7 ( n = 8–12 independent sections per group). Scale bars: 50 μm. ( K – M ) WT B6 mice were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting 72 hours after TBI. Representative images, ileal crypt frequency and height, and Ki67 + cell frequency ( n = 8–23 independent sections per group) on day 7. Scale bars: 50 μm. ( N – Q ) B6-into-BALB/c transplantation of BM with or without T cells. Recipients were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting on day 7 after BMT. Ileal crypt frequency and height and Ki67 + and Olfm4 + cell frequency ( n = 14–31 independent sections per group) on day 14 after BMT. Scale bars: 50 μm. Data are representative of at least 2 independent experiments or were combined from 2 experiments ( K – Q ). * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed t test or 1-way ANOVA.
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    ( A ) Representative Western blot of SI organoids treated with or without MP (10 μM) for 24 hours, followed by treatment with <t>rmIL-22</t> (5 ng/mL) for 2 hours. ( B – D ) Representative images and frequency and size of SI organoids cultured with or without MP and rmIL-22 (0.5 ng/mL) for 5 days ( n = 3–4 wells per group). Scale bars: 200 μm. ( E and F ) Representative images and size of human SI organoids cultured with or without MP (10 μM) and rhIL-22 (10 ng/mL) for 6 days ( n = 6 wells per group). Scale bars: 1,000 μm. ( G and H ) qPCR to determine Cdkn1a and Ccna2 expression in organoids cultured with or without MP (10 μM) and rmIL-22 (1 ng/mL) for 3 days ( n = 3 wells per group). ( I and J ) B6 mice treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day). Representative images and ileal crypt frequency and height on day 7 ( n = 8–12 independent sections per group). Scale bars: 50 μm. ( K – M ) WT B6 mice were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting 72 hours after TBI. Representative images, ileal crypt frequency and height, and Ki67 + cell frequency ( n = 8–23 independent sections per group) on day 7. Scale bars: 50 μm. ( N – Q ) B6-into-BALB/c transplantation of BM with or without T cells. Recipients were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting on day 7 after BMT. Ileal crypt frequency and height and Ki67 + and Olfm4 + cell frequency ( n = 14–31 independent sections per group) on day 14 after BMT. Scale bars: 50 μm. Data are representative of at least 2 independent experiments or were combined from 2 experiments ( K – Q ). * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed t test or 1-way ANOVA.
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    Image Search Results


    ( A ) Representative Western blot of SI organoids treated with or without MP (10 μM) for 24 hours, followed by treatment with rmIL-22 (5 ng/mL) for 2 hours. ( B – D ) Representative images and frequency and size of SI organoids cultured with or without MP and rmIL-22 (0.5 ng/mL) for 5 days ( n = 3–4 wells per group). Scale bars: 200 μm. ( E and F ) Representative images and size of human SI organoids cultured with or without MP (10 μM) and rhIL-22 (10 ng/mL) for 6 days ( n = 6 wells per group). Scale bars: 1,000 μm. ( G and H ) qPCR to determine Cdkn1a and Ccna2 expression in organoids cultured with or without MP (10 μM) and rmIL-22 (1 ng/mL) for 3 days ( n = 3 wells per group). ( I and J ) B6 mice treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day). Representative images and ileal crypt frequency and height on day 7 ( n = 8–12 independent sections per group). Scale bars: 50 μm. ( K – M ) WT B6 mice were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting 72 hours after TBI. Representative images, ileal crypt frequency and height, and Ki67 + cell frequency ( n = 8–23 independent sections per group) on day 7. Scale bars: 50 μm. ( N – Q ) B6-into-BALB/c transplantation of BM with or without T cells. Recipients were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting on day 7 after BMT. Ileal crypt frequency and height and Ki67 + and Olfm4 + cell frequency ( n = 14–31 independent sections per group) on day 14 after BMT. Scale bars: 50 μm. Data are representative of at least 2 independent experiments or were combined from 2 experiments ( K – Q ). * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed t test or 1-way ANOVA.

    Journal: The Journal of Clinical Investigation

    Article Title: Corticosteroids impair epithelial regeneration in immune-mediated intestinal damage

    doi: 10.1172/JCI155880

    Figure Lengend Snippet: ( A ) Representative Western blot of SI organoids treated with or without MP (10 μM) for 24 hours, followed by treatment with rmIL-22 (5 ng/mL) for 2 hours. ( B – D ) Representative images and frequency and size of SI organoids cultured with or without MP and rmIL-22 (0.5 ng/mL) for 5 days ( n = 3–4 wells per group). Scale bars: 200 μm. ( E and F ) Representative images and size of human SI organoids cultured with or without MP (10 μM) and rhIL-22 (10 ng/mL) for 6 days ( n = 6 wells per group). Scale bars: 1,000 μm. ( G and H ) qPCR to determine Cdkn1a and Ccna2 expression in organoids cultured with or without MP (10 μM) and rmIL-22 (1 ng/mL) for 3 days ( n = 3 wells per group). ( I and J ) B6 mice treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day). Representative images and ileal crypt frequency and height on day 7 ( n = 8–12 independent sections per group). Scale bars: 50 μm. ( K – M ) WT B6 mice were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting 72 hours after TBI. Representative images, ileal crypt frequency and height, and Ki67 + cell frequency ( n = 8–23 independent sections per group) on day 7. Scale bars: 50 μm. ( N – Q ) B6-into-BALB/c transplantation of BM with or without T cells. Recipients were treated or not with MP (2 mg/kg i.p. daily) with or without F-652 (100 μg/kg s.c. every other day), starting on day 7 after BMT. Ileal crypt frequency and height and Ki67 + and Olfm4 + cell frequency ( n = 14–31 independent sections per group) on day 14 after BMT. Scale bars: 50 μm. Data are representative of at least 2 independent experiments or were combined from 2 experiments ( K – Q ). * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed t test or 1-way ANOVA.

    Article Snippet: Cell cultures were treated with MP (Tocris), DEX (Tocris), or budesonide (Tocris) as well as rmIFN-γ (R&D Systems), rmIL-22 (R&D Systems), ruxolitinib (Selleckchem), or Stattic (Tocris).

    Techniques: Western Blot, Cell Culture, Expressing, Transplantation Assay